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NAD+ 500mg

Research Specifications

Full Name:
Nicotinamide adenine dinucleotide (oxidised form)
CAS Number:
53-84-9
Molecular Weight:
663.43 Da
Form:
Lyophilized powder
Purity:
≥98% (HPLC verified)
Storage:
−20°C; protect from moisture and light
Reconstitution:
Sterile saline or bacteriostatic water

NAD+ 500mg

Nicotinamide adenine dinucleotide — the universal electron carrier. Central to cellular energy metabolism, DNA repair, and sirtuin-mediated longevity pathways.

50%

NAD+ Decline by Age 60

Yoshino et al., Cell Metab 2018

7

Sirtuin Isoforms Activated

SIRT1-7, NAD+-dependent

663 Da

Molecular Weight

NAD+ free acid

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≥98% Purity Verified Free Shipping $200+ HPLC Tested

Electron Transfer, Sirtuin Activation & DNA Repair

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found in every living cell. It serves as the primary electron acceptor in glycolysis and the TCA cycle, shuttling electrons to the mitochondrial electron transport chain to generate ATP. Beyond energy metabolism, NAD+ is the obligate substrate for sirtuins (SIRT1-7) — NAD+-dependent deacetylases that regulate gene expression, mitochondrial biogenesis (via PGC-1α), and DNA damage responses. It is also consumed by PARP enzymes during DNA base excision repair. NAD+ levels decline ~50% between ages 20 and 60, making it a key target in longevity and metabolic research.

Research Highlights

Mitochondrial Biogenesis

NAD+ supplementation activates SIRT1, which deacetylates and activates PGC-1α — the master regulator of mitochondrial biogenesis. Research in aged mice shows NAD+ precursor supplementation increases mitochondrial mass and oxidative capacity in skeletal muscle, partially reversing age-associated metabolic decline.

DNA Damage Repair via PARP

PARP-1 (poly ADP-ribose polymerase) is the primary DNA repair enzyme for single-strand breaks, consuming ~100 NAD+ molecules per break repaired. In high-DNA-damage contexts (UV exposure, oxidative stress), PARP activation can deplete cellular NAD+ — supplementation supports repair capacity.

Synergy with GLP-1 Research

NAD+ precursors and GLP-1 agonists show complementary metabolic effects in rodent models. NAD+ supplementation enhances insulin sensitivity through SIRT1/PGC-1α, potentially potentiating the metabolic benefits of tirzepatide and retatrutide in combined protocol research.

Research FAQ

Why does NAD+ decline with age?
NAD+ biosynthesis decreases with age while NAD+ consumption by PARP (DNA repair) and CD38 (inflammatory signaling) increases. The net result is a ~50% reduction in tissue NAD+ between ages 20-60. This decline correlates with reduced mitochondrial function, impaired DNA repair, and decreased sirtuin activity.
What is the difference between NAD+ and NMN/NR?
NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are NAD+ precursors — they are converted to NAD+ intracellularly. Direct NAD+ IV/SC supplementation bypasses the conversion step and provides immediate substrate availability, making it the choice for acute cellular NAD+ repletion in research protocols.
Does NAD+ complement GLP-1 peptide protocols?
Research suggests complementarity: GLP-1 agonists reduce caloric intake and improve insulin sensitivity at the receptor level; NAD+ supports the downstream mitochondrial machinery for energy substrate utilization. SIRT1 activation by NAD+ also promotes fat oxidation via PGC-1α, potentially enhancing the fat-preferential weight loss profile of retatrutide and tirzepatide.
How should NAD+ 500mg be stored and used in research?
Store lyophilized powder at −20°C, protected from moisture and light. NAD+ is hygroscopic — exposure to humidity accelerates degradation. Reconstitute in sterile saline or bacteriostatic water immediately before use. Use within 24 hours of reconstitution for maximal potency.

Research Use Only. This product is intended for in-vitro laboratory research only. Not for human consumption, injection, or therapeutic use. Not medical advice. Always consult applicable regulations in your jurisdiction.

Price Comparison

SupplierPurityPriceShipping
Apollo (via ClavTides)>98% HPLC$69.99Free over $200
Generic Research SuppliersVaries (often <95%)Similar–HigherVaries
Pharmaceutical (Rx only)Pharmaceutical gradeNot available for researchRx required

NAD+ 500mg

$69.99

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